Introduction

Ub/Ubl deubiquitylases (DUBs) play key regulatory roles in a variety of disease states including cancer, diabetes, and infectious disease. As such, they represent an attractive class of novel targets for therapeutic development. Approximately 100 Ub/Ubl deubiquitylases have been identified in the human genome, however relatively few have been fully characterized. LifeSensors’ offers a wide range of HTS amenable assays and substrates for characterizing these proteases and discovering modulators of their activity. 

We offer two microarrays specific to DUB function that allow you to characterize and learn more about your DUB or DUB substrate.

1)     DUB ID – Determine DUBs that deubiquitylate your substrate.

2)     DUB Substrate ID – Identify substrates for your DUB.

  • Two ‘fully’ ubiquitylated protein microarrays are generated using a cocktail of E2 and E3 enzymes that ensures ubiquitylation of candidate proteins
  • One array is de-ubiquitylated using your DUB
  • Arrays are probed with ubiquitin detection reagents (antibodies or TUBEs)
  • The difference in ubiquitylation pattern between these arrays indicates substrates

 

The service can be performed with one of several commercially available arrays or a custom array.  LifeSensors can advise on the selection of the most appropriate array for the experiment.

 

CHOP Reporter System

 
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CHOP2 Reporter System

 
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Diubiquitin IQF Substrates

 
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DU0101
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Ubiquitin and Mutant Ubiquitins

 
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Ubiquitin Chains

 
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Ubiquitin and Ubiquitin-like AMC

 
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Ubiquitin and Ubiquitin-like Rhodamine

 
SI230
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Ubiquitin and Ubiquitin-like Aminoluciferins

 
SI260
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