The conjugation of ubiquitin to substrates is a multi-step, multi-enzyme process. LifeSensors is committed to supplying high throughput, facile, and robust assays for measuring the activity of Ub conjugating enzymes. These assays work with a wide range of E3 ligases including members of the three major classes of E3 ligases (simple RING class, SCF RING class, and HECT class). Our E3LITE Customizable Ubiquitin Ligase Kit, as well as the E2 profiling kit based on it, was developed in collaboration with and licensed from Progenra, Inc. (www.progenra.com).
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