The VU-1 antibody from LifeSensors is the best anti-ubiquitin antibody that we have found after trying more than ten different anti-ubiquitin antibodies. Please click here for his paper comparing VU-1 to other antibodies.
We have tried the SUMOStar technology on one of the toughest proteins in our mammalian expression platform portfolio, which previously failed to express in various expression vectors in HEK293 cells. The SUMO fusion approach resulted in an astounding yield of 3 mg of the purified recombinant protein per Litre. Due to the conformation of the fusion protein (it was a tight dimer), we experienced difficulties when trying to remove the SUMO tag. We therefore contacted the Lifesensors’ Technical Support. The feedback was remarkably speedy, expertly and very helpful: our problem was resolved. Excellent technology. Excellent product support.
We consistently use your products because of the quality and purity in comparison to other companies and because your scientists take the time to help us troubleshoot technical obstacles we encounter in our research.
The TUBE products from LifeSensors are a valuable set of tools for any lab investigating ubiquitin post-translational modifications. Their reagents make it easy to look at ubiquitin post-translational modifications on our proteins of interest. We use the TUBE products to immunoprecipitate ubiquitylated proteins from cell lysates and then perform a Western blot. Since we can use specific antibodies that we have previously optimized for Western blot, there are no additional optimization steps and the results look great. I would definitely recommend the TUBE products from LifeSensors to other investigators looking for a fast and economical way to look at specific ubiquitin post-translational modifications.