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E3 ID, Ubiquitin E3 Ligase Identification Service | Catalog #: MA101
With over 700 putative ubiquitin E3 ligases, knowledge of the E3s capable of ubiquitylating your substrate is necessary information for a thorough understanding of protein biochemistry and also proper drug target identification. E3 ID™ employs a high-content microarray and LifeSensors' ubiquitin pathway expertise to identify candidate E3's that are capable of ubiquitylating your substrate. The service includes the following steps:
The service can be performed with one of several commercially available arrays or a custom array. LifeSensors can advise on the selection of the most appropriate array for the experiment. Screens have been performed successfully with protein arrays containing approximately 20,000 human proteins and over 495 E3's.
Array: Client-selected protein microarray
Client material required: Client's substrate (roughly 150 µL of a 100 nM solution), detection antibody for client's substrate or tag (optional). Direct labeling of the protein is available.
Deliverable: Raw data & statistical analysis, follow-up support and lead prioritization
Turnaround: 4-6 weeks
Contact our scientists for a free consultation. Find out how LifeSensors' Ubiquitin Protein Microarray Services can provide the answers to your ubiquitome-related questions.
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