SUMO Protease 2

Product Information

Storage/Stability

Documentation

Product Protocol

Product Description

SUMO Protease 2, a highly active and robust recombinant protease, cleaves huSUMO3 from recombinant fusion proteins. Unlike thrombin, EK, or TEV protease, which recognize short, linear sequences, SUMO Protease 2 recognizes the tertiary structure of huSUMO3.  As a result, SUMO Protease 2 never cleaves within the fused protein of interest. SUMO Protease 2 cleaves consistently over a broad range of temperature (30°C is optimal), pH [5.5 – 9.5], and ionic strength. SUMO Protease 2 contains a polyhistidine tag at the N-terminus, allowing easy removal from the cleavage reaction by affinity chromatography.

Benefits

• High activity.
• High specificity; does not cleave within protein of interest.
• Cleavage yields native protein with a desired N-terminus (except Proline).
• Active from 2°C to 37°C

Unit Definition

One unit of SUMO Protease 2 cleaves 100 µg of HuSUMO3-MetGFP in 1h at 30°C.

Storage/Stability

Store at -80°C, avoiding repeated freeze-thaw cycles. Stable for one year under these conditions.