Proteases

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SUMO Protease 1

Catalog Number
4010
Molecular Weight
31 kDa
Family
Cysteine Protease
Buffer
Tris Buffer
Physical State
Liquid
Purity
>99%
Alternate Name(s)
Ulp1 core
Fusion Tag
His6
Concentration
10 U/μl
Source
E. coli

Product Information

Price

$315.00 USD

Please Choose:

Storage/Stability

Documentation

Product Protocol

Product Description

LifeSensors' industry leading SUMO Protease 1 (Ulp1), a highly active and robust recombinant protease, cleaves SUMO  from recombinant fusion proteins. Unlike thrombin, EK, or TEV protease, which recognize short linear sequences, SUMO Protease 1 recognizes the tertiary structure of SUMO.  As a result, SUMO Protease 1 never cleaves within the fused protein of interest. SUMO Protease 1 cleaves consistently over a broad range of temperature (30°C is optimal), pH [5.5 – 9.5], and ionic strength. SUMO Protease 1 contains a polyhistidine tag at the N-terminus, making it easy to remove from the cleavage reaction by affinity chromatography.

 
Benefits

  • High activity.
  • High specificity; does not cleave within the protein of interest.
  • Cleavage yields native protein with a desired N-terminus (except Proline).
  • Active from 2°C to 37°C


Unit Definition


One unit of SUMO Protease 1 cleaves 100 µg of SUMO-MetGFP in 1h at 30°C.

Storage/Stability

Store at -80°C, avoiding repeated freeze-thaw cycles. Stable for up to one year under these conditions..