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TECHNICAL DOCUMENTATION

Title Type Last Updated
Peptides & Proteins Expressed via SUMO/SUMOstar systems Table 10.23.2012

SUMO technologies at-a-glance Slides 01.16.2012

Ubiquitin pathway research tools at-a-glance Slides 03.13.2012

Introduction to the SUMOpro system White Paper 11.30.2009

Introduction to the SUMOstar system for BEVS White Paper 11.04.2008

Introduction to the secretory SUMOstar system for mammalian cell expression White Paper 09.23.2009

Introduction to the intracellular SUMOstar system for mammalian cell expression White Paper 09.23.2009

PR-619 for the inhibition of Ubiquitin and UBL-isopeptidases White Paper 08.26.2010

Using SUMO for the Enhancement of Secretion in P. pastoris White Paper 07.05.2011

pE-SUMOpro Manual, Prokaryote (Cat. No. 1000) Manual 09.06.2011

pE-SUMOpro3 Manual, Prokaryote (Cat. No. 1010) Manual 09.06.2011

pE-SUMOstar Manual, Prokaryote (Cat. No. 1100) Manual 09.06.2011

pP-SUMOpro3 Manual, Pichia (Cat. No. 2150) Manual 10.01.2010

pY-SUMOstar Manual, Saccharomyces (Cat. No. 2100) Manual 01.05.2010

pI-SUMOstar Manual, Insect (Cat. No. 3100) Manual 04.18.2011

pM-SUMOstar Manual, Mammalian (Cat. No. 7110/7120) Manual 09.23.2009

SUMO Protease 1 Protocol (Cat. No. 4010) Manual 11.05.2010

SUMO Protease 2 Protocol (Cat. No. 4020) Manual 10.01.2010

SUMOstar Protease Protocol (Cat. No. 4110) Manual 11.05.2010

IQF-DiUb Manual Manual 10.26.2011

Ubiquitin CHOP1 Manual (PR1001) Manual 12.16.2009

Ubiquitin CHOP2 Manual (PR1101) Manual 12.16.2009

SUMO3 CHOP1 Manual (PR1002) Manual 12.16.2009

SUMO3 CHOP2 Manual (PR1102) Manual 12.16.2009

Smt3 CHOP1 Manual (PR1003) Manual 12.16.2009

NEDD8 CHOP1 Manual (PR1004) Manual 12.16.2009

NEDD8 CHOP2 Manual (PR1104) Manual 11.22.2011

ISG15 CHOP1 Manual (PR1005) Manual 12.16.2009

ISG15 CHOP2 Manual (PR1105) Manual 12.16.2009

GST/His6 TUBE Manual Manual 11.23.2010

Agarose TUBE Manual Manual 11.23.2010

Biotin TUBE Manual Manual 11.23.2010

E3Lite Customizable Ubiquitin Ligase Kit Manual 01.25.2012

E2 Profiling Kit Manual 11.08.2010

Biotin TUBE for Far Western blot App Note 11.08.2010

K48-linked Diubiquitin Assay App Note 11.08.2010

K63-linked Diubiquitin Assay App Note 11.08.2010

E3Lite for high throughput screening App Note 11.08.2010

E2-E3 cognate pair determination App Note 11.08.2010

SUMO system, a universal platform for protein expression, purification and function Poster 05.09.2011

Enhanced expression, purification and enzymatic characterization of USP20 produced in insect cells Poster 08.23.2010

Enhanced expression and secretion of SUMOstar fusion proteins in the flashBAC GOLD BEVS Poster 05.09.2011

Physical and kinetic characterization of a novel IQF deubiquitylase substrate for measuring the cleavage of K63 linked ubiquitin chains Poster 08.23.2010

Use of an internally quenched fluorescence-diubiquitin (IQF-DiUb) based substrate to measure isopeptide bond cleavage: Application to high throughput screening Poster 08.23.2010

Identification of novel chemical space using innovative deubiquitylase assays Poster 07.08.2011

Bioluminescence assay platform for selective and sensitive detection of Ub/Ubl proteases Poster 07.11.2011

Deubiquitylase, deSUMOylase, and DeISGylase activity microarrays for assay of substrate preference and functional modifiers Poster 08.23.2010

E3 and DUB substrate identification and polyubiquitin chain linkage specificity profiling using microarrays Poster 08.23.2010

E3 and DUB substrate identification using microarrays. Poster 12.13.2011

Development of a sandwich ELISA for the quantification of total ubiquitin (mono- + poly- Ub) in biological samples Poster 07.11.2011

Deubiquitylation and protein aggregation Implications for neurodegenerative disease Poster 12.19.2011

Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels Paper 10.14.2011

Deubiquitylase, deSUMOylase, and deISGylase activity microarrays for assay of substrate preference and functional modifiers Paper 10.26.2011

Activity-Based Chemical Proteomics Accelerates Inhibitor Development for Deubiquitylating Enzymes Paper 12.19.2011

Integrative analysis of the ubiquitin proteome isolated using Tandem Ubiquitin Binding Entities (TUBEs) Paper 05.30.2012

Accelerated Production of Subunit Vaccine Antigens Using SUMO Fusion Tags and Single-use Bioreactors Poster 05.03.2012

A microarray of ubiquitylated proteins for profiling deubiquitylase activity reveals the critical roles of both chain and substrate Paper 05.30.2012

Decoding the information content of ubiquitin chains: Application of linkage-specific TUBES to the ubiquitome Poster 06.25.2012

Effect of DUB inhibitor PR-619 on the microtubule network and protein aggregate formation in oligodendroglial cells: Implications for neurodegeneration Poster 06.25.2012
LifeSensors News

HEADLINES

LifeSensors News
 
LifeSensors presented an overview of ubiquitin proteomics at the 3rd GTC Conference, Ubiquitin Research and Drug Discovery, Las Vegas, February 25-26th, 2013.
Complexity of ubiquitin pathways leads to frequent questions related to identification of ubiquitin pathway enzymes (E3s and DUBs) and their substrates. An overview of biochemical proteomics for drug discovery applications including Ubiquitin Protein Microarray Services from LifeSensors was presented by Christian Loch, Assistant Director, R&D. Visit our website to find additional information about our Ubiquitin Protein Microarrays.
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LifeSensors

LifeSensors Inc. appointed 4A Biotech Co.Ltd as another distributor of its innovative reagents and technologies in China
Located at Guanglian Industrial Park, Beijing, 4A Biotech Co.Ltd. is a leading life science product provider in China.
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LifeSensors

LifeSensors presents data at FASEB's Ubiquitin & Cellular Regulation meeting June 24th-29th.
Download PDFs of poster #54 "Effect of DUB inhibitor PR-619 on the microtubule network and protein aggregate formation in oligodendroglial cells: Implications for neurodegeneration" and poster #61 "Decoding the information content of ubiquitin chains: Application of linkage-specific TUBES to the ubiquitome" from our Technical Documentation page (bottom of the page).
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