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LifeSensors is a company driven by innovation and, as such, has received multiple SBIR grants from the NIH. Most of our products and technologies have come out of in-house research and development projects. We strongly believe in disseminating knowledge by publication of our key findings. Over the last ten years, LifeSensors scientists have authored or co-authored a number of publications on these technologies. We invite you to request copies of our papers and publications.
Yan, Y., Strickler, JE, and Butt TR., SUMO Fusion Technology for Enhanced Protein Expression and Purification in Prokaryotes and Eukaryotes. , Humana Press (in press), 2010
Yan, Y., Orcutt, SJ, and Strickler JE., SUMO Fusion System for Protein Expression and Purification. , Chemistry Today. (in press), 2010
Hughes, S.R., Rich, J.O., Kenneth M Bischoff, K. M., Hector, R. E., Qureshi, S., Saha, B.D, Dien, B. S., Liu, S., Cox; E.J., Jackson, J.S., Sterner, D. E., Butt, T. R, LaBaer, J., Cotta, M., Automated Yeast Transformation Protocol to Engineer Saccharomyces cerevisiae Strains for Cellulosic Ethanol Production with Open Reading Frames That Express Proteins Binding to Xylose isomerase Identified Using a Robotic Two-Hybrid Screen. , J. Assoc. Lab. Autom. (in press), 2009
Hughes, S.R.; Hector, R.E.; Rich, J.O.; Qureshi, N.; Bischoff, K.M.; Dien, B.S.; Saha, B.C.; Liu, S.; Jackson, J.S. Jr.; Sterner, D.E.; Butt, T.R.; LaBaer, J.; Cotta, M.A., Automated yeast mating protocol using open reading frames from Saccharomyces cerevisiae genome to improve yeast strains for cellulosic ethanol production. , J. Assoc. Lab. Autom. (in press), 2009
Peroutka, R.J., Elshourbagy, N., T.L. Piech, TL. and Butt, TR, Enhanced protein expression in mammalian cells using engineered SUMO fusions: Secreted phospholipase A2. , Prot. Sci., 2008. 17:1586-1595.
Liu, L., Spurrier, J, Butt, TR and Strickler, JE, Enhanced protein expression in the baculovirus/insect cell system using engineered SUMO fusions. , Prot. Exp. Purif., 2008. 62: 21-28.
Hughes, S. R., Sterner, D.E., Hector, R. E., Bischoff, K. M., Dowd, P. F., Bang, S. S., Grynavyski, N., Chakrabarty, T., Qureshi, N., Johnson, E. T., Dien, B. S., Liu, S., Caughey, R. J., LaBaer, J., Butt, T. R., Michael A Cotta, M. A., Rich, J.O., Three-Plasmid SUMO Yeast Vector System for Automated High-Level Functional Expression of Value- Added Co-Products in a Saccharomyces cerevisiae Strain Engineered for Xylose Utilization. , Plasmid., 2008. 61:22-38.
Hughes SR, Dowd PF, Hector RE, Panavas T, Sterner DE, Qureshi N, Bischoff KM, Bang SS, Mertens JA, Johnson ET, Li XL, Jackson JS, Caughey RJ, Riedmuller SB, Bartolett S, Liu S, Rich JO, Farrelly PJ, Butt TR, Labaer J, Cotta MA. ., Lycotoxin-1 insecticidal peptide optimized by amino acid scanning mutagenesis and expressed as a coproduct in an ethanologenic Saccharomyces cerevisiae strain. , J Pept Sci., 2008. 9:1039-50.
Panavas, T, Sanders C, F and Butt, T. R., SUMO fusion technology for enhanced protein production in prokaryotic and eukaryotic expression systems. , In Methods in Molecular Biology: SUMO Protocols, Ullrich, H, ed. Humana Press, 2008. pp 303-317
Nicholson, B., Leach, Craig A, Goldenberg, Seth J, Francis, Dana M, Kodrasov, Matthew P, Tian, Xufan, Shanks, John, Sterner, David E, Bernal, Alejandro, Mattern, Michael R, Wilkinson, Keith D, and Butt, Tauseef R., Characterization of Ubiquitin and Ubiquitin-like Protein Isopeptidase Activities. , Protein Science, 2008. 17: 1035-1043
Nicholson, B., Marblestone, J.G., Butt, T. R., and Mattern, M. R., Deubiquitinating Enzymes as Novel Anticancer Targets. , Future Oncology, 2007. 3: 191-199
Hughes, S.R., Dowd, P.F., Hector, R.E., Riedmuller, S.B., Bartolett, S., Mertens, J.A., Qureshi, N., Liu, S., Bischoff, K. M., Li, X-L., Jackson, Jr, J.S., Sterner, D., Panavas, T., Rich, J., Farrelly, P.J., Butt, T.R., Cotta, M.J., High-Throughput Multiplex Library Construction of Synthetic Mutagenized Insecticidal Peptides Using a Fully Automated Plasmid-Based Functional Proteomic Robotic Workcell with an Improved Vacuum Block. , J Assoc Lab Automation J. Assoc. Lab. Autom., 2007. 12(4):2002-2012
Arnold, JJ, Bernal, A, Uche, U, Sterner, DE, Butt, T. R, Cameron, CE, and Mattern, MR., SUMO isopeptidase assay based on poliovirus RNA polymerase activity. , Analytical Biochem, 2006. 350: 214-221
Marblestone JG, Edavettal SC, Lim Y, Lim P, Zuo X, Butt TR., Comparison of SUMO fusion technology with traditional gene fusion systems: Enhanced expression and solubility with SUMO. , Protein Science, 2006. 2006 15:182-9.
Hall J., Applying Fusion Protein Technology to E. coli. , BioPharm International, 2007.
Hall J, Mattern MR, Butt TR., Mining the Ubiqitin Pathway. , The Scientist, 2005. 19(23):42-43.
Butt TR, Edavettal SC, Hall JP, Mattern MR., SUMO fusion technology for difficult-to-express proteins. , Protein Expr Purif., 2005. 43(1):1-9.
Zuo X, Li S, Hall J, Mattern MR, Tran H, Shoo J, Tan R, Weiss SR, Butt TR., Enhanced expression and purification of membrane proteins by SUMO fusion in Escherichia coli. , J Struct Funct Gen, 2005. 6:103-11.
Hall J., Production of Difficult-to-Express Proteins. , Genetic Engineering News, 2005. 25(9):52-53.
Zuo X, Mattern MR, Tan R, Li S, Hall J, Sterner DE, Shoo J, Tran H, Lim P, Sarafianos SG, Kazi L, Navas-Martin S, Weiss SR, Butt TR., Expression and purification of SARS coronavirus proteins using SUMO-fusions. , Protein Expr Purif., 2005. 42(1):100-10.
Malakhov MP, Mattern MR, Malakhov OA, Drinker M, Weeks SD, Butt TR., SUMO fusions and SUMO-specific protease for efficient expression and purification of proteins. , J Struct Funct Gen., 2004. 5:75-86.
Hjerpe R, Aillet F, Lopitz-Otsoa F, Lang V, England P, Rodriguez MS., Efficient protection and isolation of ubiquitylated proteins using tandem ubiquitin-binding entities. , EMBO Rep., Pub Med: 2009 October 2 (In Press)
