EXPRESSION SYSTEMS
Insect Intracellular Expression
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SUMOstar Technology LifeSensors has developed SUMOstar to enhance the production fusion proteins, while resisting the proteolytic activity of endogenous SUMO proteases. SUMOstar fusion constructs are not processed in vivo, allowing for the same enhancement of protein expression, solubility, and stability afforded by SUMOpro. For specific recognition and cleavage of SUMOstar fusions in vitro, LifeSensors has also developed SUMOstar protease 1. With the exception of proline, SUMOstar protease 1 will cleave your fusion protein precisely between the C-terminus of SUMOstar and the N-terminus of the target protein. By recognizing the tertiary structure of the SUMOstar tag, this system virtually eliminates the ragged ends and non-specific cleavage often seen with other proteases. SUMOstar Technology for Baculoviral Expression Vector Systems (BEVS) Everything you need for pilot studies and initial scale up of BEVS expression of SUMOstar fusion proteins is together in a convienent kit format. If intracellular expression of the POI is desired, pI-SUMOstar is your choice for over-expression in a BEVS. However, if you require secretion from the cell, the use of pI-secSUMOstar will target your POI for processing and secretion to the media. |
