Our SUMO-based Protein and Peptide Expression Systems maximize the yield of soluble, functional proteins in E. coli, yeast, insect and mammalian cells. This system utilizes unique features of SUMO functioning as both a chaperonin and as an initiator of protein folding to dramatically improve the solubility and level of expression of the protein of interest. Our desumoylases efficiently and robustly remove the SUMO tag precisely at the junction of the C-terminus of SUMO and the N-terminus of the protein releasing it with the desired N-terminal amino acid (with the exception of proline). Both SUMO and the desumoylases have His6 tags making their subsequent removal fast and easy.

Which system is right for you?

• Prokaryotic expression (E. coli) Choose from any of our SUMO tags, SUMO-Pro, SUMOstar, or SUMO-Pro3.  SUMOstar and SUMO-Pro3 serve as entry points to future eukaryotic systems.

• Expression in yeast (S. cerevesiae or P. pastoris) choose our yeast SUMOstar or SUMO-Pro3 systems.

• Eukaryotic systems (insect and mammalian cell) choose our SUMOstar system.

Each system is available in a convenient kit format containing all necessary reagents or as individual components.