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Expression Systems


Add your protein of interest to the growing bibliography of SUMO-expressed proteins!

Our SUMO-tag expression systems maximize the yield of soluble, functional proteins in E. coli, yeast, insect and mammalian cells. This system features SUMO functioning as both a chaperonin and as an initiator of protein folding to dramatically improve the solubility and level of expression of your protein of interest. Our desumoylases efficiently and precisely remove SUMO tags, releasing your protein with the desired N-terminal amino acid. Both the SUMO tags and the desumoylases have His6 tags making their subsequent removal fast and easy.

Enhanced Expression and Solubility Using SUMO vs. Other Commonly-used Fusion Tags



Figure legend. GDF8 (myostatin), a secreted TGFβ family member and difficult-to-express protein, was expressed in E. coli either as a His6, Ub, SUMO, MBP, GST, TRX, or NUS A fusion. Protein fractions were resolved by SDS-PAGE and stained with Coomassie. Arrowheads indicate expected/observed positions of respective protein bands. Of all tags tested, SUMO was the only fusion tag that provided high-level expression of soluble protein.


Express your protein the first time and every time with SUMO. Choose your preferred expression system below and get started with SUMO today!


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Expression Systems

E. Coli

Bacterial expression is the usual starting point for expression of heterologous proteins. Bacterial fermentations are inexpensive and can reach high cell densities resulting in high volumetric yields of the target protein. Our SUMO fusion technology is ideally suited for this system.
Expression Systems

Mammalian

Mammalian cell expression has become the system of choice for production of complex, glycosylated biotherapeutic proteins such as antibodies, growth factors and fertility hormones. Our SUMOstar system has been designed for transient transfection studies in HEK293 or CHO cells.
Expression Systems

Insect

Baculovirus/insect cell systems have found wide application for the expression of highly recalcitrant proteins such as protein tyrosine kinases. We have developed a SUMOstar-based system to support expression studies in this system.
Expression Systems

Yeast

As with bacterial expression systems, yeast offer relatively inexpensive growth media and high density fermentation. Furthermore, yeast offer the capability of carrying out limited post-translational modifications such as disulfide bond formation and glycosylation. We offer two yeast expression systems, one for intracellular expression in S. cerevisiae and the second for extracellular expression in P. pastoris.
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HEADLINES

LifeSensors News
 
LifeSensors Awarded U.S. Patent for Novel Ubiquitin Pathway Enzyme Substrates
LifeSensors, Inc., a biotech company developing cutting-edge technologies for studying the ubiquitin pathway, has been awarded a U.S. patent for the development of novel, fluorescent di- and poly-ubiquitin deubiquitinase (DUB) substrates (US 8,518,660).
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Human SUMO-3 Patent Expands LifeSensors' Protein Expression Portfolio
Malvern, PA, USA — March 7, 2012. LifeSensors, Inc., developer and manufacturer of SUMO-fusion technology used to overcome bottlenecks in protein production, expands its intellectual property and protein expression portfolio with its patent, “Human SUMO-3 For Enhancing Protein Expression” (US 8,119,369 B2), awarded by the United States Patent and Trademark Office last week.
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LifeSensors' SUMO-fusion technology
Update from PEGS 2011 meeting
MALVERN, PA – BOSTON, MA- May 25th. LifeSensors’ Protein Expression platform based on Small Ubiquitin Modifier (SUMO) fusion technology, received a lot of attention at the Seventh Annual Protein Engineering Summit (PEGS) May 9-13, 2011 in Boston, MA.
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