LIFESENSORS :: EXPRESSION SYSTEMS - YEAST SECRETORY EXPRESSION (PICHIA): PP-PICHIA SECRETORY SUMOPRO3 EXPRESSION KIT

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Yeast Secretory Expression (Pichia)

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pP-Pichia Secretory SUMOpro3 Expression Kit

Catalog #: 2150

pP-Pichia Secretory SUMOpro3 Expression Kit

Catalog #:

2150

UNIT SIZE:
QUANTITY:

Prices are for U.S. and Canada. Please contact the LifeSensors distributor in your country for pricing.

SUMOpro3 Fusion Protein Technology.

The use of SUMOpro3 as a fusion system can dramatically enhance overall protein production through enhanced protein translation, solubility and stability. Each SUMO-fusion system requires a specific protease for removal of the fusion partner -- for SUMOpro3 use SUMO Protease 2. SUMO proteases are highly specific and robust enzymes that recognize and bind to the tertiary structure of SUMO in order to correctly position the C-terminal cleavage site in the enzymes active site. With the exception of proline, these proteases will cleave your fusion protein precisely between the C-terminus of SUMO and the N-terminus of the target protein. This requirement for the tertiary structure of SUMO virtually eliminates the ragged ends and non-specific cleavage seen with other proteases.


Fusion Tag:	His6-hSUMO3
Promoter:	AOX1
Host:	P. pastoris
DNA:	Circular

APPLICATIONS BENEFITS DOCUMENTATION INFO

APPLICATIONS
Enhancing functional protein expression IN P. Pastoris

BENEFITS
The benefits of the SUMOpro®-3 Expression System:

Human SUMO3 fusion often enhances expression
Human SUMO3 fusion often enhances protein solubility
No known case of SUMO Protease 2 cleaving within the fused protein-of-interest
Cleavage yields native protein with a desired N-terminus

DOCUMENTATION

PRODUCT INFORMATION
Components:

pE-secSUMOpro3 vector (Cat.# 2151) -- 20 ug
SUMO Protease 2 (Cat.# 4020) -- 500 Units
SUMO Protease 2 control protein (Cat.# 5004) -- 100 µg
Anti-hSUMO3 antibody (Cat.# AB7030) -- 50 µg

Storage/stability:

Store vector components at -20°C
Avoid repeated freeze-thaw cycles
Store protease and antibody components at -80°C

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610.644.8845

International: +1.610.644.8845 or email us directly.

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