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EXPRESSION SYSTEMS - E. Coli
Protease

SUMO Proteases

Also found here: SUMOstar Protease | SUMOstar Protease | SUMO Proteases
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SUMOstar Protease 1

Catalog #: 4110
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  SUMOstar Protease 1
Catalog #: 4110

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Recombinant SUMOstar Protease 1 specifically recognizes and cleaves the SUMOstar tag from recombinant fusion proteins. Unlike thrombin, EK, or TEV proteases, which recognize short, linear sequences, SUMOstar Protease 1 recognizes the tertiary structure of SUMOstar.  As a result, SUMOstar Protease  1 will not cleave within the protein of interest. To cleave the SUMOpro tag (orginial yeast SUMO tag), please use SUMO Protease 1

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Molecular weight: 26 kDa
Fusion tag: His6
Source: E. coli
Purity: ≥95%
Physical state: Liquid
Activity: ≥105 Unit/mg

APPLICATIONS
SUMOstar Protease 1 contains a polyhistidine tag at the N-terminus, allowing easy removal from the cleavage reaction by affinity chromatography.  The protease cleaves consistently over a broad range of temperature (30°C is optimal), pH [5.5 – 9.5], and ionic strength.
PRODUCT INFORMATION
  • Specifically engineered to recognize and cleave the SUMOstar fusion tag. 
  • Activity: 1U of SUMOstar Protease 1 cleaves >90% of 100 ug SUMOstar Protease 1 Control Protein in 1hour (30°C)
  • Storage/stability:  Store at -80°C, avoiding repeated freeze-thaw cycles.
  • Stable for up to one year under these conditions.

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LifeSensors presented an overview of ubiquitin proteomics at the 3rd GTC Conference, Ubiquitin Research and Drug Discovery, Las Vegas, February 25-26th, 2013.
Complexity of ubiquitin pathways leads to frequent questions related to identification of ubiquitin pathway enzymes (E3s and DUBs) and their substrates. An overview of biochemical proteomics for drug discovery applications including Ubiquitin Protein Microarray Services from LifeSensors was presented by Christian Loch, Assistant Director, R&D. Visit our website to find additional information about our Ubiquitin Protein Microarrays.
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LifeSensors Inc. appointed 4A Biotech Co.Ltd as another distributor of its innovative reagents and technologies in China
Located at Guanglian Industrial Park, Beijing, 4A Biotech Co.Ltd. is a leading life science product provider in China.
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LifeSensors presents data at FASEB's Ubiquitin & Cellular Regulation meeting June 24th-29th.
Download PDFs of poster #54 "Effect of DUB inhibitor PR-619 on the microtubule network and protein aggregate formation in oligodendroglial cells: Implications for neurodegeneration" and poster #61 "Decoding the information content of ubiquitin chains: Application of linkage-specific TUBES to the ubiquitome" from our Technical Documentation page (bottom of the page).
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