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EXPRESSION SYSTEMS - E. Coli
Expression Kits

LifeSensors offers three types of SUMO expression systems in E. coli. All three systems are built on a pET24 vector with T7 promoters, and are available with either ampicillin or kanamycin resistance selectable markers.

SUMOpro is the original yeast SUMO, which enhances the expression and solubility of your expressed protein. SUMOpro only works in prokaryotic systems, and is cleaved from your protein by SUMO Protease 1.

SUMOstar is a version of yeast SUMO that has been engineered for use in both prokaryotic and eukaryotic cells. Use SUMOstar to enhance expression and solubility of your protein in E. coil, and retain the ability to clone your SUMOstar-fusion into yeast, insect/BEVS and mammalian vectors for future eukaryotic expression. SUMOstar is cleaved from your protein by SUMOstar Protease 1.   

SUMOpro3 is based on the human SUMO-3 protein which is resistant to desumoylases found in eukaryotic cells. Use SUMOpro3 to enhance expression and solubility of your protein in E. coil, and retain the ability to clone your SUMOpro3-fusion into yeast (Pichia pastoris) for future eukaryotic expression. SUMOpro3 is cleaved from your protein by SUMO Protease 2.

The Most Cost-Effective SUMO Protease  
Manufacturers: Life Technologies (Invitrogen) LifeSensors
Unit (u) definition at 30°C Amount of enzyme to cleave 85% of 2µg of protein per hour Amount of enzyme to cleave 90% of 100µg of protein per hour
Cost for 250 units $335.00 $175.00
Tag removal cost $778/mg cleaved protein $7.78/mg cleaved protein



 

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Expression Systems
Expression Systems
PRODUCT NAME CATALOG#        PRICE (USD) QTY
Expression Systems
SUMOpro3 Kit     More Information »
pE-SUMOpro3 Amp Kit 1010A
pE-SUMOpro3 Kan Kit 1010K
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LifeSensors presented an overview of ubiquitin proteomics at the 3rd GTC Conference, Ubiquitin Research and Drug Discovery, Las Vegas, February 25-26th, 2013.
Complexity of ubiquitin pathways leads to frequent questions related to identification of ubiquitin pathway enzymes (E3s and DUBs) and their substrates. An overview of biochemical proteomics for drug discovery applications including Ubiquitin Protein Microarray Services from LifeSensors was presented by Christian Loch, Assistant Director, R&D. Visit our website to find additional information about our Ubiquitin Protein Microarrays.
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LifeSensors Inc. appointed 4A Biotech Co.Ltd as another distributor of its innovative reagents and technologies in China
Located at Guanglian Industrial Park, Beijing, 4A Biotech Co.Ltd. is a leading life science product provider in China.
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LifeSensors presents data at FASEB's Ubiquitin & Cellular Regulation meeting June 24th-29th.
Download PDFs of poster #54 "Effect of DUB inhibitor PR-619 on the microtubule network and protein aggregate formation in oligodendroglial cells: Implications for neurodegeneration" and poster #61 "Decoding the information content of ubiquitin chains: Application of linkage-specific TUBES to the ubiquitome" from our Technical Documentation page (bottom of the page).
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