LIFESENSORS :: EXPRESSION SYSTEMS - SUMOPRO KIT: PE-SUMOPRO KAN KIT

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EXPRESSION SYSTEMS - E. Coli
Expression Kits

SUMOpro Kit

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pE-SUMOpro Kan Kit

Catalog #: 1000K

pE-SUMOpro Kan Kit

Catalog #:

1000K

UNIT SIZE:
QUANTITY:

Prices are for U.S. and Canada. Please contact the LifeSensors distributor in your country for pricing.

The SUMOpro Protein and Peptide Expression Kit maximizes yields of soluble, untagged, functional proteins with the desired N-terminus. While other prokaryotic expression systems use engineered protease cleavage sites that produce amino acid extensions and often result in non-specific cleavage within the protein of interest, LifeSensors’ highly active and specific SUMO protease 1 recognizes the tertiary structure of the SUMO tag, generating the desired N-terminus and never cleaving within your protein of interest.

In addition, the SUMOpro kit makes purifying your protein affordable and straightforward. By linking a His6 epitope to both the SUMO fusion tag and SUMO Protease 1, the SUMOpro system allows efficient and rapid purification of untagged protein using immobilized metal affinity chromatography.


Fusion Tag:	His₆-SUMO
Promoter:	T7 RNA polymerase
Selection marker:	Kanamycin
Host:	E. coli
DNA:	Circular

APPLICATIONS BENEFITS DOCUMENTATION INFO

APPLICATIONS
High-yield production of untagged recombinant proteins in E. coli

BENEFITS

SUMOpro may dramatically enhance the solubility of recombinant proteins in E. coli

Specific cleavage of SUMO yields native protein with desired N-terminus (except proline)

SUMOpro has been shown to protect peptides from intracellular degradation

DOCUMENTATION

PRODUCT INFORMATION
Components:

pE-SUMO Kan vector (Cat.# 1001K) -- 20 µg
SUMO Protease 1 (Cat.# 4010) -- 500 Units
SUMO Protease 1 control protein (Cat.# 5000) -- 100 µg
Anti-SUMO/SUMOstar antibody (Cat.# 7002) -- 50 µg

Storage/stability:

Store vector components at -20°C
Avoid repeated freeze-thaw cycles
Store protease and antibody components at -80°C

To speak with a scientist about your specific requirements or to order over the phone,
please call...

610.644.8845

International: +1.610.644.8845 or email us directly.

LifeSensors presented an overview of ubiquitin proteomics at the 3rd GTC Conference, Ubiquitin Research and Drug Discovery, Las Vegas, February 25-26th, 2013.
Complexity of ubiquitin pathways leads to frequent questions related to identification of ubiquitin pathway enzymes (E3s and DUBs) and their substrates. An overview of biochemical proteomics for drug discovery applications including Ubiquitin Protein Microarray Services from LifeSensors was presented by Christian Loch, Assistant Director, R&D. Visit our website to find additional information about our Ubiquitin Protein Microarrays.
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LifeSensors Inc. appointed 4A Biotech Co.Ltd as another distributor of its innovative reagents and technologies in China
Located at Guanglian Industrial Park, Beijing, 4A Biotech Co.Ltd. is a leading life science product provider in China.
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LifeSensors presents data at FASEB's Ubiquitin & Cellular Regulation meeting June 24th-29th.
Download PDFs of poster #54 "Effect of DUB inhibitor PR-619 on the microtubule network and protein aggregate formation in oligodendroglial cells: Implications for neurodegeneration" and poster #61 "Decoding the information content of ubiquitin chains: Application of linkage-specific TUBES to the ubiquitome" from our Technical Documentation page (bottom of the page).
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