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EXPRESSION SYSTEMS - E. Coli
Antibody

LifeSensors offers three types of SUMO expression systems in E. coli. All three systems are built on a pET24 vector with T7 promoters, and are available with either ampicillin or kanamycin resistance selectable markers.

SUMOpro is the original yeast SUMO, which enhances the expression and solubility of your expressed protein. SUMOpro only works in prokaryotic systems, and is cleaved from your protein by SUMO Protease 1.

SUMOstar is a version of yeast SUMO that has been engineered for use in both prokaryotic and eukaryotic cells. Use SUMOstar to enhance expression and solubility of your protein in E. coil, and retain the ability to clone your SUMOstar-fusion into yeast, insect/BEVS and mammalian vectors for future eukaryotic expression. SUMOstar is cleaved from your protein by SUMOstar Protease 1.   

SUMOpro3 is based on the human SUMO-3 protein which is resistant to desumoylases found in eukaryotic cells. Use SUMOpro3 to enhance expression and solubility of your protein in E. coil, and retain the ability to clone your SUMOpro3-fusion into yeast (Pichia pastoris) for future eukaryotic expression. SUMOpro3 is cleaved from your protein by SUMO Protease 2.

The Most Cost-Effective SUMO Protease  
Manufacturers: Life Technologies (Invitrogen) LifeSensors
Unit (u) definition at 30°C Amount of enzyme to cleave 85% of 2µg of protein per hour Amount of enzyme to cleave 90% of 100µg of protein per hour
Cost for 250 units $335.00 $175.00
Tag removal cost $778/mg cleaved protein $7.78/mg cleaved protein



 

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PRODUCT NAME CATALOG#        PRICE (USD) QTY
Expression Systems
Antibodies     More Information »
Anti-hSUMO3 antibody AB7030
Anti-SUMO/SUMOstar antibody AB7002
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LifeSensors is a proud sponsor of the 5th Annual Ubiquitin Drug Discovery & Diagnostics Conference
This year's conference will be held at the Four Seasons Hotel in Philadelphia, July 22-24 and will once again highlight the latest research taking place in the ubiquitin field. We are excited to learn about all of the latest discoveries and technical advances and hope to see you there!
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LifeSensors

LifeSensors presented an overview of ubiquitin proteomics at the 3rd GTC Conference, Ubiquitin Research and Drug Discovery, Las Vegas, February 25-26th, 2013.
Complexity of ubiquitin pathways leads to frequent questions related to identification of ubiquitin pathway enzymes (E3s and DUBs) and their substrates. An overview of biochemical proteomics for drug discovery applications including Ubiquitin Protein Microarray Services from LifeSensors was presented by Christian Loch, Assistant Director, R&D. Visit our website to find additional information about our Ubiquitin Protein Microarrays.
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LifeSensors

LifeSensors Inc. appointed 4A Biotech Co.Ltd as another distributor of its innovative reagents and technologies in China
Located at Guanglian Industrial Park, Beijing, 4A Biotech Co.Ltd. is a leading life science product provider in China.
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